Phytochemicals, Cytotoxicity, Inhibitory Effect on Nitric Oxide Production and Alpha-Glucosidase Activity, and DPPH Scavenging Activity of Leaf Extract from Gymnema inodorum (Lour.) Decne

Investigation of phytochemicals, cyto-toxicity, inhibitory effect on nitric oxide (NO) production and α-glucosidase activity, DPPH scavenging activity leaf extract from Gymnema inodorum (Lour.) Decne (LEGI) revealed that GC-MS analysis demonstrated the presence 83 phytochemicals with 5 main compounds i.e. Phytol (18.14 %), n-Hexadecanoic acid (12.45 9,12-Octadecadienoic (Z,Z)-(3.92 9,12,15-Octadecatrienoic acid, (Z,Z,Z)-(10.39 %) Betulin (8.56 78 trace in LEGI. MTT assay to investigate cytotoxicity showed LEGI exhibited low RAW 264.7 macrophages IC50 128.77±2.82 µg/mL. Treatment significantly inhibited production, an inflammatory mediator, LPS-stimulated cells. At concentration 50 mg/mL, NO by 16.73±1.52 % which was less than Diclofenac did (27.07±1.81 %). The determine antidiabetic inhibition 0.34 potent Acarbose (IC50 0.20 mg/mL). Assay 2,2-diphenyl-2-picrylhydrazyl- hydrate (DPPH) radical examine antioxidant compared Butylated hydroxytoluene, BHT 0.43 vs 0.36 respectively. These results indicate G. possesses anti-inflamatory, activities can be developed used as natural agents for tréamatent diseases related these activities. HIGHLIGHTS Using analysis, there are identified Phytol, (Z,Z)-, (Z,Z,Z)- Betulin, ethanol inodorum, is most predominant compound inhibit indicating its anti-inflammatory property displays α-Glucosidase suggesting applied management evidenced free radicals shows µg/ml, GRAPHICAL ABSTRACT